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Annals of Occupational Hygiene Advance Access originally published online on September 20, 2004
Annals of Occupational Hygiene 2004 48(7):601-606; doi:10.1093/annhyg/meh052
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© British Occupational Hygiene Society Published by Oxford University Press;

Reliability of a Visual Scoring System with Fluorescent Tracers to Assess Dermal Pesticide Exposure

AURORA ARAGÓN1,2,*, LUIS BLANCO1,2, LYLLIAM LÓPEZ1, CAROLA LIDÉN3, GUN NISE2 and CATHARINA WESSELING2,4

1 Department of Preventive Medicine and Public Health, Faculty of Medicine, National Autonomous University of Nicaragua, León, Nicaragua; 2 Department of Public Health Sciences, Division of Occupational Health, Karolinska Institutet, Stockholm, Sweden; 3 Department of Medicine, Occupational and Environmental Dermatology, Karolinska Institutet and Stockholm County Council, Sweden; 4 Central American Institute for Studies on Toxic Substances, Universidad Nacional, Heredia, Costa Rica

* Author to whom correspondence should be addressed. e-mail: aragon{at}unanleon.edu.ni

Received 2 January 2004; in final form 16 April 2004

Background and objectives: We modified Fenske's semi-quantitative ‘visual scoring system’ of fluorescent tracer deposited on the skin of pesticide applicators and evaluated its reproducibility in the Nicaraguan setting.

Methods: The body surface of 33 farmers, divided into 31 segments, was videotaped in the field after spraying with a pesticide solution containing a fluorescent tracer. A portable UV lamp was used for illumination in a foldaway dark room. The videos of five farmers were randomly selected. The scoring was based on a matrix with extension of fluorescent patterns (scale 0–5) on the ordinate and intensity (scale 0–5) on the abscissa, with the product of these two ranks as the final score for each body segment (0–25). Five medical students rated and evaluated the quality of 155 video images having undergone 4 h of training. Cronbach alpha coefficients and two-way random effects intraclass correlation coefficients (ICC) with absolute agreement were computed to assess inter-rater reliability.

Results: Consistency was high (Cronbach alpha = 0.96), but the scores differed substantially between raters. The overall ICC was satisfactory [0.75; 95% confidence interval (CI) = 0.62–0.83], but it was lower for intensity (0.54; 95% CI = 0.40–0.66) and higher for extension (0.80; 95% CI = 0.71–0.86). ICCs were lowest for images with low scores and evaluated as low quality, and highest for images with high scores and high quality.

Conclusions: Inter-rater reliability coefficients indicate repeatability of the scoring system. However, field conditions for recording fluorescence should be improved to achieve higher quality images, and training should emphasize a better mechanism for the reading of body areas with low contamination.

Keywords: fluorescent tracer • Nicaragua • pesticides • reliability • skin exposure • visual scoring system


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