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Ann. occup. Hyg., Vol. 46, No. suppl_1, pp. 31-34, 2002
© 2002 British Occupational Hygiene Society
Published by Oxford University Press

In Vivo and In Vitro Expression of CYP1A1 in Rat Epithelial Cells After Instillation of Quartz

Andrea Becker*, Catrin Albrecht, Roel Schins, AD Knaapen, Doris Höhr, Klaus Unfried and Paul J. A. Borm

Institute of Environmental Research, Particle Toxicology Auf'm Hennekamp 50, D-40225 Düsseldorf, Germany

*Author to whom correspondence should be addressed.

Cytochrome P450 monooxygenases have been suggested to be altered after exposure to quartz and to play a role in quartz-induced lung cancer. The aim of the study was to investigate expression of CYP1A1 in vivo in isolated epithelial cells from Wistar rats and in vitro in the rat lung epithelial cell line RLE after exposure to DQ12. CYP1A1 mRNA after acute (3 days) and subchronic (90 days) exposure to quartz (2 mg) was evaluated in vivo in comparison with in vitro examinations. Although a modification of CYP1A1 activity in rat lung homogenates was demonstrated by other authors, we could not measure any alteration in mRNA expression in epithelial cells after exposure to quartz. Further investigations will study the participation of other cell types and their role in xenobiotic metabolism after chronic quartz exposure.

CYP1A1 • cytochrome P450 • DQ12 • quartz • rat lung epithelial cells • RLE • xenobiotic metabolism


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